Regulation of the circadian system by HCN3 channels
Circadian rhythms are controlled by a master clock located in the suprachiasmatic nucleus of the hypothalamus (SCN). The intergeniculate leaflet (IGL) is critical for synchronizing this clock to day-night rhythm via projections to the SCN. In IGL neurons the hyperpolarization-activated current (Ih) is essential for intrinsic excitability and rhythmic burst firing. There is initial evidence that HCN3 channels underlie this current. In this proposal we will use a HCN3 deficient mouse line which was generated in our lab to characterize the specific role of HCN3 channels in the IGL for the regulation of circadian rhythm.