Visualizing changes in neuronal connectivity during memory formation in mouse neocortex
We aim to identify structural and functional correlates of declarative memory formation in mouse neocortex. We will train mice on visual memory tasks, probing the animal’s ability to form visual pair associates. Two-photon calcium imaging will reveal how neurons in visual cortex alter their responses during learning. Concurrent structural imaging allows assessing whether these changes correlate with modifications in synaptic inputs at the level of individual neurons. Finally, we will employ a combined in vivo – in vitro approach to assess changes in the connectivity of functionally characterized neurons using dual patch recordings and circuitry mapping with glutamate uncaging and optogenetics.